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Structured Review

Addgene inc h2b rfp
H2b Rfp, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 77 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/lv+rfp+plasmid/LV-RFP+(Plasmid+%2326001)/pm42778593-564-19-20
Average 94 stars, based on 77 article reviews
h2b rfp - by Bioz Stars, 2026-10
94/100 stars

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Related Articles

Knockdown:

Article Title: In vivo single-cell CRISPR screening for microproteins identifies a critical ribosomal component
Article Snippet: .. To knock down Rpl41 in nontargeting and Gm10076 C7 mutant keratinocytes, 3 different shRNAs were cloned into a modified LV RFP plasmid (Addgene #26001), where H2B-RFP was replaced with a blasticidin selection marker. .. First, the Pfl23II restriction site was inserted before H2B RFP via a Phusion mutagenesis Polymerase reaction (Thermo Scientific, F530S).

Mutagenesis:

Article Title: In vivo single-cell CRISPR screening for microproteins identifies a critical ribosomal component
Article Snippet: .. To knock down Rpl41 in nontargeting and Gm10076 C7 mutant keratinocytes, 3 different shRNAs were cloned into a modified LV RFP plasmid (Addgene #26001), where H2B-RFP was replaced with a blasticidin selection marker. .. First, the Pfl23II restriction site was inserted before H2B RFP via a Phusion mutagenesis Polymerase reaction (Thermo Scientific, F530S).

Clone Assay:

Article Title: In vivo single-cell CRISPR screening for microproteins identifies a critical ribosomal component
Article Snippet: .. To knock down Rpl41 in nontargeting and Gm10076 C7 mutant keratinocytes, 3 different shRNAs were cloned into a modified LV RFP plasmid (Addgene #26001), where H2B-RFP was replaced with a blasticidin selection marker. .. First, the Pfl23II restriction site was inserted before H2B RFP via a Phusion mutagenesis Polymerase reaction (Thermo Scientific, F530S).

Modification:

Article Title: In vivo single-cell CRISPR screening for microproteins identifies a critical ribosomal component
Article Snippet: .. To knock down Rpl41 in nontargeting and Gm10076 C7 mutant keratinocytes, 3 different shRNAs were cloned into a modified LV RFP plasmid (Addgene #26001), where H2B-RFP was replaced with a blasticidin selection marker. .. First, the Pfl23II restriction site was inserted before H2B RFP via a Phusion mutagenesis Polymerase reaction (Thermo Scientific, F530S).

Plasmid Preparation:

Article Title: In vivo single-cell CRISPR screening for microproteins identifies a critical ribosomal component
Article Snippet: .. To knock down Rpl41 in nontargeting and Gm10076 C7 mutant keratinocytes, 3 different shRNAs were cloned into a modified LV RFP plasmid (Addgene #26001), where H2B-RFP was replaced with a blasticidin selection marker. .. First, the Pfl23II restriction site was inserted before H2B RFP via a Phusion mutagenesis Polymerase reaction (Thermo Scientific, F530S).

Selection:

Article Title: In vivo single-cell CRISPR screening for microproteins identifies a critical ribosomal component
Article Snippet: .. To knock down Rpl41 in nontargeting and Gm10076 C7 mutant keratinocytes, 3 different shRNAs were cloned into a modified LV RFP plasmid (Addgene #26001), where H2B-RFP was replaced with a blasticidin selection marker. .. First, the Pfl23II restriction site was inserted before H2B RFP via a Phusion mutagenesis Polymerase reaction (Thermo Scientific, F530S).

Marker:

Article Title: In vivo single-cell CRISPR screening for microproteins identifies a critical ribosomal component
Article Snippet: .. To knock down Rpl41 in nontargeting and Gm10076 C7 mutant keratinocytes, 3 different shRNAs were cloned into a modified LV RFP plasmid (Addgene #26001), where H2B-RFP was replaced with a blasticidin selection marker. .. First, the Pfl23II restriction site was inserted before H2B RFP via a Phusion mutagenesis Polymerase reaction (Thermo Scientific, F530S).



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